ABSTRACT
A cultura de calos de Gomphrena globosa a partir de segmentos foliares foi estabelecida em meio contendo combinações dos fitoreguladores auxina/citocinina. Metabólitos secundários identificados através da fitoquímica da planta in natura, foram utilizados como padrões em análises por cromatografia líquida de alta eficiência e cromatografia de gás de extratos da cultura. O estudo comparativo revelou que os calos foram capazes de produzir alguns metabólitos sintetizados pela planta in natura.
The culture of calluses of Gomphrena globosa starting from segments foliate was established in half containing combinations of the phytoregulators auxina/citocinina. Identified secondary metabolites through the phytochemistry of the plant in natura were used as patterns in analyses for high performance liquid cromatography and gas cromatography of extracts of the culture. The comparative study revealed that the calluses were capable to produce some metabolite synthesized by the plant in natura.
ABSTRACT
Seminal vesicle reactivity to cholinergic agents, plasma testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) conmcentrations and seminal vesicle testosterone concentrations were determined in adult male rats treated during the first 6 h of life with 1.0 ml peanut oil (oil-treated), 1.0 mg testosterone propionate (TP-treated) or 1.2 mg 19-nor-testosterone homofarnesate (19-NT-treated). At 90-100 days of age, the neonatally treated animals presented atrophied accessory genital organsand increased (TP-treated, N=10) or unchanged (19-NT-treated, N=11) pD, values for acetylcholine (vehicle; 5.18 ñ 0.06, N=10; TP-treated; 5.26 ñ 0.06, N+10; 19-NT-treated; 5.14 ñ 0.09, , N=11), and acetyyl-beta-methylcholine (vehicle: 5.19 ñ 0.07; TP-treated: 5.43 ñ 0.06; 19-NT-treated 5.25 ñ 0.07). The relative intrinsic activity, alpha, of acetyl-beta-methylcholine increased after both hormonal treatments (vehicle: 0.85 ñ 0.03; TP-treated:0.95 ñ 0.02; 19-NT-treated: 0.92 ñ 0.03). No variation in mean adult plasma testosterone concentration was observed after neonatal treatmentwith either TP or 19-NT (vehicle:752.93 ñ 273.66, N+8; TP-treated; 459.05 ñ 88.32, N=8; 19-NT-treated: 836.86 ñ 113.08, N=7). However, testosterone content of seminal vesicles of adult rats was decreased in the animalstreated with TP (N=5) and 19-NT (N=6) compared to controls. These results indicate a specific effect of neonatal hormone treatment on androgen metabolism which is demonstrable in the adult
Subject(s)
Rats , Animals, Newborn/therapy , Gonadal Steroid Hormones , Parasympathomimetics/metabolism , Seminal Vesicles , Acetylcholine , TestosteroneABSTRACT
The influence of hormones on the submandibular gland of rodents has attracted more attention since the observation of the sexual dimorphism of these organs. Androgens enhance both the development and secretory activity of the gland. In the present investigation we have studied the differences in wet weight, protein content, glandulain activity and morphometry of granular convoluted tubules of submandibular glands excised from control, castrated and antiandrogen-teated (RU 23908 or cyproterone acetate) male adult albino rats. Castration and antiandrogenic treatment did not affect the wet weight or protein content of the organs. Castration or treatment with RU 23908, but not treatment with cyproterone acetate, decreased glandulain activity and the height of the secretory epithelium of granular ducts. The morphology and glandulain activity of submandibular gland granular ducts do not seem to be related solely to plasma testosterone concentration. Different hormonal treatments are needed to identify other factors implicated in the phenomenon